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Resonance assignment and structural studies of larger proteins by nuclear magnetic resonance (NMR) can be challenging when exchange broadening, multiple stable conformations, and 1H back-exchange of the fully deuterated chain pose problems. These difficulties arise for the SARS-CoV-2 Main Protease, a homodimer of 2 × 306 residues. We demonstrate that the combination of four-dimensional (4D) TROSY-
The 68-kDa homodimeric 3C-like protease of SARS-CoV-2, Mpro (3CLpro/Nsp5), is a promising antiviral drug target. We evaluate the concordance of models generated by the newly introduced AlphaFold2 structure prediction program with residual dipolar couplings (RDCs) measured in solution for 15N-1HN and 13C′-1HN atom pairs. The latter were measured using a new, highly precise TROSY-AntiTROSY Encoded R
Understanding the factors that underpin the enormous catalytic proficiencies of enzymes is fundamental to catalysis and enzyme design. Enzymes are, in part, able to achieve high catalytic proficiencies by utilizing the binding energy derived from nonreacting portions of the substrate. In particular, enzymes with substrates containing a nonreacting phosphodianion group coordinated in a distal site
Types and functions of roles in work groups are presented in overview followed by a discussion of relations between roles, personality and team roles. Role conflicts, role stress and problems in roles are discussed and advice is given on how to apply small group role theory to group work in higher education and the chapter ends with suggestions for exercises and a few role related instruments.
Despite that climate hazards are increasingly felt across the globe, there is widespread and often subtle resistance to climate adaptation interventions. However, adaptation research and practice have largely focused on overcoming barriers to implementation. By presuming adaptation programs are welcome, they miss that many people oppose or refuse to participate in them, and the politics hidden beh
Today’s digitalized society relies on the advancement of silicon (Si) Complementary Metal Oxide Semiconductor (CMOS) technology, but the limitations of down-scaling and the rapidly increasing demand for added functionality that is not easily achieved in Si, have pushed efforts to monolithically 3D-integrate III-V devices above the Si-CMOS technology. In addition, the demand for increased computati
The reactivity of oxide surfaces is often attributed to formation and annihilation of oxygen deficient sites, which undergo complex structural rearrangements in the process. A prototypical example is VOx/TiO2 catalysts, widely used for e.g. selective catalytic reduction (SCR) of NOx emissions, where the possible effect of the supporting TiO2 surface structure has been much debated. Here, we presen
Titanium-aluminium nitride (Ti1-xAlxN) coatings are used to achieve high oxidation and wear resilience in coated metal cutting tools. For the highest performance, the coatings' composition and microstructure are carefully controlled. However, it is difficult to obtain knowledge how these factors affect of the oxidation processes at the microstructural level because studies are most commonly perfor
The use of ETEM in oxidation studies of carbon nanostructures such as CB has revealed new, and confirmed previously suggested, oxidation mechanisms. However, the influence of the electron beam on the dynamic oxidation processes must be well understood in order to connect the results to the real world. To date, there are few studies reporting any quantification of the effect1,2. To better understa
This chapter questions the rallying cry that ‘waste is a resource’ through the lens of François Jullien’s critique of efficacy. Focusing on the waste hierarchy, the lean movement, and the circular economy, it shows that viewing waste as a resource privileges a heroic mode of action based on ideals, means and ends, and intended outcomes. Contrasting ancient Greek and Chinese philosophies, Jullien c
The dispersion scan (d-scan) technique is extended to measurement of the time-dependent polarization state of ultrashort laser pulses. In the simplest implementation for linearly polarized ultrashort pulses, the d-scan technique records the second harmonic generation spectrum as a function of a known spectral phase manipulation. By applying this method to two orthogonally polarized projections of
BACKGROUND: Precision medicine has the potential to improve cardiovascular disease (CVD) risk prediction in individuals with Type 2 diabetes (T2D).METHODS: We conducted a systematic review and meta-analysis of longitudinal studies to identify potentially novel prognostic factors that may improve CVD risk prediction in T2D. Out of 9380 studies identified, 416 studies met inclusion criteria. Outcome
Det här är en roman; den femte av mina kriminalromaner om Thierry av Liège, vilka utspelar sig under Tyska ordens innehav av Gotland 1398-1408.
The US-Japan alliance established over seventy years ago is typically described by leaders on both sides as the ‘cornerstone of peace and security in East Asia.’ While China and North Korea may not share this positive view of the impact of the alliance on the region, the fact is that few alliances in modern international relations rival the US-Japan one in terms of durability, integration and infl
The blurred distinction between freedom of expression and hate speech in ever more polarised public debates across Europe and beyond has prompted research on hate speech, particularly focusing on right-wing populist politicians. Little is known, however, about how this distinction is construed by ordinary citizens. Deploying the concept of retrogressive mobilisation, this study examines how cases
In atrial fibrillation (AF), autonomic nervous system (ANS) differences among individuals may have a substantial influence on the varying effectiveness of anti-AF treatments. This work aimed to assess the relationship between changes in autonomic balance and in atrial fibrillatory rate (AFR) oscillations (F_{mathrm{f}}(t)) induced by head-up (HUT) and head-down (HDT) tilt test. 22 persistent AF (p
The conduction properties of the atrioventricular (AV) node have a significant impact on heart rate during permanent atrial fibrillation (AF), and can be modulated through the use of β-blockers or calcium channel blockers. These drugs have different physiological effects and are often selected empirically. Hence, an improved understanding of how these drugs affect the AV node conduction properties
Water stress can severely decrease crop productivity by restricting photosynthesis, while the use of plastic film mulching can mitigate these water stress effects. However, the intricacies of photosynthetic and stomatal responses to soil water stress under plastic film mulching, particularly when combined with atmospheric water stress, have not been well studied, especially in arid irrigation area
