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We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a
We investigated the impact of inertial forces on acoustic sorting at high sample throughputs. Above a critical flow rate, we observed an unexpected inertia induced breakdown of the sorting efficiency that is not due to the wall induced lift force and was not previously described in the literature. We analyzed the effect experimentally and theoretically, providing an explanation to the phenomena, a
This chapter addresses potential political implications of social media by looking into everyday appropriations of the political opportunities offered to active citizens. It presents and analyses data from interviews with 33 Swedish online participating citizens to gain insight into how they work both with separate and in-between (cross) internet applications as part of their political participati
Enligt författarnas mångåriga erfarenhet från kustprojekt runt om i Sverige föreligger ett stort behov av en förbättrad terminologi på svenska vad gäller ingenjörsmässiga frågeställningar och planering av kustområden.Föreliggande artikel presenterar således en engelsk-svensk ordlista, inklusive förklarande kommentarer, med syfte att klargöra en lång rad ord och uttryck. Denna finns också att tillg
The identification of helium nuclei at LHCb is achieved using a method based on measurements of ionisation losses in the silicon sensors and timing measurements in the Outer Tracker drift tubes. The background from photon conversions is reduced using the RICH detectors and an isolation requirement. The method is developed using pp collision data at √s = 13 TeV recorded by the LHCb experiment in th
How do we relate to monuments and statues in public spaces? And how do our perceptions shift when history confronts us with new insights?In the wake of the Black Lives Matter movement in 2020, many monuments and statues across the world became the focal point of intense debate. Some were torn down, others covered up, and others were re-examined in light of not only colonial and racial oppression,
We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known
