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Efficient characterization of retro-, lenti-, and foamyvector-transduced cell populations by high-accuracy insertion site sequencing

The identification of unknown genomic flanking DNA sequences can be used for the molecular monitoring of retro-, lenti- and foamyviral integration, transgenes in early embryogenesis, insertional mutagenesis, cell fate, and stem cell plasticity. Most existing methods reflect shortcomings in sensitivity and or specificity, thus limiting genomic sequencing of unknown flanking DNA to clonal preparatio

Analysis of regulatory phosphorylation sites in ZAP-70 by capillary high-performance liquid chromatography coupled to electrospray ionization or matrix-assisted laser desorption ionization time-of-flight mass spectrometry

The adapter protein APS associates with the multifunctional docking sites Tyr-568 and Tyr-936 in c-Kit.

The adapter protein APS has previously been shown to be involved in recruiting the ubiquitin E3 ligase c-Cbl to the insulin receptor, the platelet-derived growth factor b-receptor and the erythropoietin receptor, leading to increased degradation of the receptors and inhibition of mitogenesis. Here we demonstrate, by use of immobilized synthetic phosphopeptides corresponding to various autophosphor

Dynamics of water molecules in the active-site cavity of human cytochromes P450

Identification of Csk tyrosine phosphorylation sites and a tyrosine residue important for kinase domain structure

Redox potentials of the blue copper sites of bilirubin oxidases

Does nest site availability limit the density of hole nesting birds in small woodland patches?

Geometry, Reduction Potential, and Reorganization Energy of the Binuclear CuA Site, Studied by Density Functional Theory

Effects of substitutions in the CXXC active-site motif of the extracytoplasmic thioredoxin ResA

Ground-Based Optical Measurements at European Flux Sites: A Review of Methods, Instruments and Current Controversies

Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases

Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. We found that TFKs phosphorylate preferential

Modelling changes in forest soil chemistry at 16 Swedish coniferous forest sites following deposition reduction

Site selective dissociation upon core ionization of ozone

Sites and the mental landscape : Stone Age in the Kullen district, North-western Scania, Sweden

Persistent organochlorine pollutants in children working at a waste-disposal site and in young females with high fish consumption in Managua, Nicaragua

Experience from Two Resistivity Inversion Techniques Applied in Three Cases of Geotechnical Site Investigation

Magnetic phases of one-dimensional lattices with 2-4 fermions per site

Porphyrin Binding and Distortion and Substrate Specificity in the Ferrochelatase Reaction: The Role of Active Site Residues.

The specific insertion of a divalent metal ion into tetrapyrrole macrocycles is catalyzed by a group of enzymes called chelatases. Distortion of the tetrapyrrole has been proposed to be an important component of the mechanism of metallation. We present the structures of two different inhibitor complexes: (1) N-methylmesoporphyrin (N-MeMP) with the His183Ala variant of Bacillus subtilis ferrochelat

Organic compounds in aerosols from selected European sites - Biogenic versus anthropogenic sources

Available energy and energy balance closure at four coniferous forest sites across Europe