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beta 1 Integrin and alpha-dystroglycan binding sites are localized to different laminin-G-domain-like (LG) modules within the laminin alpha 5 chain G domain

Laminins are a group of extracellular-matrix proteins important in development and disease. They are heterotrimers, and specific domains in the different chains have specialized functions. The G domain of the alpha5 chain has now been produced in transfected mammalian cells as single modules and two tandem arrays, alpha5LG1-3 and alpha5LG4-5 (LG is laminin G domain-like.). Using these fragments we

Protein Folding Studied by Site Directed Mutagenesis

The N-terminal RNA binding domain of U1A has been shown to fold in a two-state process without accumulating any intermediates. U1A has a symmetrically curved chevron plot which seems to result mainly from changes of the transition state. At low concentrations of denaturant the transition state occurs early in the folding reaction, whereas at high denaturant concentration it moves close to the nati

Site investigation with combined methods in a faulted area in Managua, Nicaragua - a pre-study

Geophysical methods are increasingly being applied to geotechnical investigations, as they can identify material properties and material boundaries, as well as variations in space and time of relatively large volumes of soil. Another advantage is that many of these methods are non-intrusive. The combination of several methods and the verification of their results by sampling and correlating with g

Exploration of conformational flexibility and hydrogen bonding of xylosides in different solvents, as a model system for enzyme active site interactions

The predominantly populated conformation of carbohydrates in solution does not necessarily represent the biologically active species; rather, any conformer accessible without too large an energy penalty may be present in a biological pathway. Thus, the conformational preferences of a naphthyl xyloside, which initiates in vivo synthesis of antiproliferative glycosaminoglycans, have been studied by

Analysis of particle size distribution changes between three measurement sites in northern Scandinavia

We investigated atmospheric aerosol particle dynamics in a boreal forest zone in northern Scandinavia. We used aerosol number size distribution data measured with either a differential mobility particle sizer (DMPS) or scanning mobility particle sizer (SMPS) at three stations (Varrio, Pallas and Abisko), and combined these data with the HYSPLIT (Hybrid Single Particle Lagrangian Integrated Traject

Sex tourist risk behaviour - An on-site survey among Swedish men buying sex in Thailand.

Background: Thailand is not only a popular destination for Swedish tourists, it is also the foreign country where the largest numbers of Swedish males contract HIV. This study investigated sexual risk-behaviour of Swedish men who have sex with commercial sex workers (CSW) in Thailand. Method: Eligible men were approached on location in red-light districts of Pattaya and Bangkok with a self-adminis