Solubility and Stability Optimization for Painless Protein Purification
The protein Painless is an ion channel coupled receptor that is present in the mosquito Aedes aegypti transmitting yellow fever. The protein is activated by noxious heat. It has been a long-term goal to determine the protein structure and consequently be able to develop a mosquito repellant. The aims of the thesis were to purify and isolate heterologously expressed Painless from yeast cells and ex
