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Purification and proteomic analysis of plant plasma membranes.

All techniques needed for proteomic analyses of plant plasma membranes are described in detail, from isolation of plasma membranes to protein identification by mass spectrometry (MS). Plasma membranes are isolated by aqueous two-phase partitioning yielding vesicles with a cytoplasmic side-in orientation and a purity of about 95%. These vesicles are turned inside-out by treatment with Brij 58, whic

Disorders of the neck and upper limbs in women in the fish processing industry

OBJECTIVE--The aim was to study the association between personal factors and physical and psychosocial work environment factors and disorders of the neck or upper limbs among women in the fish processing industry. METHODS--A cross sectional study was performed on 206 women in the fish processing industry and 208 control women. Several physical and psychosocial work environment factors were evaluat

Ionization by pH and Anionic Surfactant Binding Gives the Same Thickening Effects of Crosslinked Polyacrylic Acid Derivatives

Physical properties of aqueous solutions of hydrophobically modified crosslinked polyacrylic acids change quite extensively as the polymer is charged up. A study is carried out concerning the similarities between two polymer ionization processes, that is, by pH increment and anionic surfactant addition. The two processes charge the polymer by distinctly different mechanisms. At sufficiently high p

Treatment of synthetic textile wastewater by homogeneous and heterogeneous photo-Fenton oxidation

The efficiencies of homogeneous and heterogeneous photo-Fenton oxidation were compared for treatment of azo dye containing synthetic textile wastewater. The influence of parameters such as the presence of NaCl and starch was evaluated and optimal iron and H2O2 dosage determined. Complete decolorization of Remazol Red RR was achieved at all investigated NaCl and starch concentration. Mineralization

Lipase-mediated synthesis of six-membered cyclic carbonates from trimethylolpropane and dialkyl carbonates: Influence of medium engineering on reaction selectivity

Six-membered cyclic carbonates are potential monomers for aliphatic polycarbonates and polyurethanes in a process without using toxic phosgene and isocyanate. Lipase catalyzed transesterification of the polyol, trimethylolpropane (TMP) with dimethyl carbonate (DMC) or diethyl carbonate (DEC) followed by thermal cyclization was used for synthesis of six-membered cyclic carbonates with pendant hydro

Local inhibition of ornithine decarboxylase reduces vascular stenosis in a murine model of carotid injury

Objectives: Polyamines are organic polycations playing an essential role in cell proliferation and differentiation, as well as in cell contractility, migration and apoptosis. These processes are known to contribute to restenosis, a pathophysiological process often occurring in patients submitted to revascularization procedures. We aimed to test the effect of alpha-difluoromethylornithine (DFMO), a

How does testing affect retrieval-related processes? - An event-related potential (ERP) study on the short-term effects of repeated retrieval

Abstract in UndeterminedThe testing effect is conceptualized as the benefit for remembering items that were studied and tested rather than just studied. Thus far, little is known about the underlying neurocognitive mechanisms. In an event-related potential (ERP) study, we investigated the immediate consequences of testing on recollection processes. During an initial study phase, participants encou

Application of multivariate virtual reference feedback tuning for wastewater treatment plant control

The objective of this paper is to apply the Virtual Reference Feedback Tuning (VRFT) to Multiple-Input Multiple-Output (MIMO) control strategies in wastewater treatment plants (WWTPs). Using the Benchmark Simulation Model No. 1 (BSM1) as a case study, the proposed data-driven approach provides reduced-order controllers only using a batch of input-output data points (i.e. manipulated variable-contr

Significance of the dimensional view for visualizing relevant aspects of a production system in a co-operative planning process

Pictorial visualization is expected to facilitate communication between industrial professionals when planning working environments and production systems. This hypothesis was investigated by studying how 24 participants including managers, supervisors, machine operators, and occupational health and safety officials, judged three types of computer animated visualization varying in dimensional view

Gene expression profiling of Escherichia coli expressing double Vitreoscilla haemoglobin

In a recent investigation, expression of a double Vitreoscilla haemoglobin (two fused VHb molecules) in Escherichia coli grown in shake flasks resulted in higher final cell density and considerably higher levels of ribosomes and tRNA. In this study, we have investigated the E. coli transcriptome in cells expressing native VHb, double VHb and control cells lacking VHb by hybridising mRNA from the d

Keeping My Ways of Being. Middle-aged women and menopause

Popular Abstract in Swedish Att fortsätta leva på mitt sätt. Medelålders kvinnor och menopaus/klimakteriet. Varje år passerar cirka 65 000 svenska kvinnor sin menopaus. Menopaus är en medicinsk term och avser tidpunkten för en kvinnas sista menstruation. Tidpunkten för menopaus kan därför i regel endast avgöras i efterhand, vanligtvis först efter ett års uppehåll av de naturliga menstruationernaTo deepen the understanding of menopause and its role in women's lives is the general aim of this thesis. Between 1995 and 2003, all women in two Swedish primary health care districts received a postal questionnaire, when they were 45, 50, 55 or 60. A total of 1681 women (76%) responded. Twenty-four women were given research interviews. The effects of menopause on quality of life (QoL) and number

Identification of a common non-apoptotic cell death mechanism in hereditary retinal degeneration.

Cell death in neurodegenerative diseases is often thought to be governed by apoptosis; however, an increasing body of evidence suggests the involvement of alternative cell death mechanisms in neuronal degeneration. We studied retinal neurodegeneration using 10 different animal models, covering all major groups of hereditary human blindness (rd1, rd2, rd10, Cngb1 KO, Rho KO, S334ter, P23H, Cnga3 KO

What speakers do and what addressees look at: Visual attention to gestures in human interaction live and on video

This study investigates whether addressees visually attend to speakers’ gestures in interaction and whether attention is modulated by changes in social setting and display size. We compare a live face-to-face setting to two video conditions. In all conditions, the face dominates as a fixation target and only a minority of gestures draw fixations. The social and size parameters affect gaze mainly w

Är förvaltningslagens dröjsmålstalan ett effektivt rättsmedel ur den enskildes perspektiv? - En undersökning av i vad mån utformingen av 12 § FL uppfyller sitt tilltänkta syfte

I samband med att den nya förvaltningslagen (2017:900) (FL) trädde i kraft 2018 så infördes en så kallad dröjsmålstalan genom 12 § FL. Syftet med bestämmelsen är att enskilda ska få ett verktyg för att kunna påskynda handläggningen av ett ärende i det fall som processen präglas av oskäliga dröjsmål. 12 § FL har dock kommit att kritiseras. Redan under remissbehandlingen påpekade ett flertal instansAs the new Administrative Procedure Act came in to force in 2018 a new remedy was introduced to counteract the inaction of administrative authorities. Its aim is to provide individuals with an opportunity to enforce a decision within four weeks when the processing of an errand has been unreasonably delayed. Already during the stage of development, the remedy was criticized for its inability to ful

Challenges and Possibilities Arising in the Development of Human Rights Indicators for State Reporting

HR indicators are increasingly used as a method for analysis in the work with human rights. However, the concept of HR indicators is often subject to confusion due to the lack of a common and universal theoretical framework. This study focuses on the elaboration of HR indicators for state reporting within the UN treaty monitoring bodies and argues that an extended and more coherent use of HR indic

Min lilla vrå bland bergen

Hidden on the plot of my summerhouse is a mountain glace overlooking the ocean. It is an enthralling place - a rock wall in the north and views towards the ocean in the south. A World War II trench is blasted into the rock, holding intimate rooms and layer upon layer of history. Here is narrow inclosure and vast openness, all at once. This mountain glace has been unused and forgotten during my who

Saknaden och samtalet – en undersökning av sorgegruppsarbetet i Växjö stifts församlingar

The purpose of this essay is to examine how bereavement groups in the Church of Sweden work in practice. I have familiarized myself with terms like grief, grief process and grief recovery, to find out what grief is. Grief can be described as a normal response to a loss of any kind. Grief is the pain and suffering which we can experience after a loss. In this phase, a bereavement group can be helpf

Ergonomic Design of a New Assembly Skid - A Case Study at a Swedish Truck Manufacturing Company

This thesis project was performed in collaboration with Scania CV AB’s cab factory in Oskarshamn, Sweden, and the objective was to accomplish a product development process regarding a new assembly skid from an ergonomic perspective. A great number of aspects had to be noticed and evaluated, both regarding the process the skid is aiming to support and the product it concerns. The focus was the func