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Insekters DNA i luften? På senare år har flera forskningsrapporter visat att olika insektgrupper drastiskt minskar i antal. Till följd av detta kan man även se att artkompositionen förändrats och att andra organismer som är beroende av insekter, antingen för pollinering eller som föda, också minskar i antal. För att kunna förstå varför dessa förändringar sker så krävs en kontinuerlig övervakning In the face of widely reported declines in insect abundance and diversity, there is a great need for faster and easier insect monitoring methods as the already established methods are time consuming and only target specific species groups. In this study I investigate if metabarcoding of eDNA samples collected from the air can i) detect and identify insect species, ii) help quantify spatial and tem

Tänk om Apples Siri kunde fylla i en blankett åt dig på jobbet. Eller om Google Assistant planerade veckans inköpslista. Tänk om alla banala sysslor som du utför rutinmässigt utan någon djupare åtanke kunde skötas automatiskt av en artificiell intelligens, AI. Hur underhållande detta scenario än får vara så är en framtid där alla har en personlig universell assistent fortfarande långt borta. Men äThe Cascade-Correlation learning algorithm, Cascor, is a self-constructive learning algorithm introduced by Fahlman in 1990, which builds the networks one node at the time based on the errors of the network. Cascor has been criticized for creating excessively deep networks and easily overfit. This thesis investigates a few methods that use an internal validation set to add nodes that perform bette

The period of 1880-1910 was a time of fast modernization and industrialization in Transylvania. Fertility decline only started in the South, while in the North there was a marked fertility increase. This paper attempts to explain these differences, using a cross-sectional analysis of fertility for 4112 Transylvanian settlements. The factors affecting fertility are modeled using the Eastrelin-Crimm

Based on articles published 1990-2004 in 21 LIS journals, a set of co-citation analyses were performed to study changes in research fronts over the last 15 years, where LIS is at now; and to discuss where it is heading. To study research fronts, here defined as current and influential co-cited papers, a citations among documents methodology was applied; and to study changes, the analyses were time

The aim of this project is to make a model which could be used during risk identification in swedish underground stations. The model is based on a risk identification in T-centralen, Stockholm and will in a foreseeable way structure the risk sources that can affect personal safety. There will be suggestions concerning preventive measures in an underground station, both material and organizational.

Genom alla tider har migration varit ett frekvent inslag i människans utveckling. Idag har globaliseringen och de teknologiska framstegen möjliggjort ett än större flöde av människor världen över. De flesta länderna i den industrialiserade världen har en svag och i vissa fall till och med en negativ befolkningstillväxt samtidigt som många av u-länderna kämpar med en stark befolkningstillväxt. Dett

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known

In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DN

Amyloid beta peptides (Aβ) proteins play a key role in vascular pathology in Alzheimer’s Disease (AD) including impairment of the blood–brain barrier and aberrant angiogenesis. Although previous work has demonstrated a pro-angiogenic role of Aβ, the exact mechanisms by which amyloid precursor protein (APP) processing and endothelial angiogenic signalling cascades interact in AD remain a largely un

Common remediation and land management approaches often preclude economically attractive and low risk brownfield redevelopment. The authors argue that recently developed dynamic site characterization techniques can greatly improve sound decision making on land revitalization options. A number of these in-situ soil and groundwater test methods, such as MIP, ROST™/UVOST™, XRF and BAT, based on geote

Floder och åar är viktiga källor för järn och andra näringsämnen till Östersjön. De senaste decennierna har järnkoncentrationen i svenska och finska åar ökat kraftigt, vilket skulle kunna leda till en ökad transport av järn till Östersjön. Vilken effekt detta kan ha för Östersjön beror på vad som händer med järnet när det möter det salta vattnet i Östersjön. Järn transporteras huvudsakligen i formRivers have traditionally not been considered important sources of bioavailable iron (Fe) to the marine waters, since most Fe is lost by salinity-induced aggregation and sedimentation during estuarine mixing. However, recent studies from boreal rivers found a remarkably high Fe stability, probably due to the interaction with organic matter. Recent studies have suggested that Fe speciation is a key

INTRODUCTION: The FEV1 quotient (FEV1Q), calculated as the index between FEV1 and a theoretical lower survivable FEV1 threshold of 0.4L for females and 0.5L for males, has been investigated as a novel method of interpreting results from lung function testing. The applicability of the FEV1Q in populations with chronic respiratory failure has not been studied, and the continuous association between

Title: The future is looking bright - Studies of burnout among musicians and music teachers. Since late 1970's, the burn-out-syndrome has been researched in several ways, especially among people working in care-oriented employments. The object with this study is to find out the biggest issues about why musicians and music teachers burn themselves out. This is based on musicians and music teach

We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dy

We present the first one-step acoustic separation of high quality plasma and mononuclear cells (MNCs) from undiluted human whole blood. This enables centrifugation-free integration in analytical instrumentation.

The identification of helium nuclei at LHCb is achieved using a method based on measurements of ionisation losses in the silicon sensors and timing measurements in the Outer Tracker drift tubes. The background from photon conversions is reduced using the RICH detectors and an isolation requirement. The method is developed using pp collision data at √s = 13 TeV recorded by the LHCb experiment in th

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a

We investigated the impact of inertial forces on acoustic sorting at high sample throughputs. Above a critical flow rate, we observed an unexpected inertia induced breakdown of the sorting efficiency that is not due to the wall induced lift force and was not previously described in the literature. We analyzed the effect experimentally and theoretically, providing an explanation to the phenomena, a