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CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a

Inertia induced breakdown of acoustic sorting efficiency at high flow rates

We investigated the impact of inertial forces on acoustic sorting at high sample throughputs. Above a critical flow rate, we observed an unexpected inertia induced breakdown of the sorting efficiency that is not due to the wall induced lift force and was not previously described in the literature. We analyzed the effect experimentally and theoretically, providing an explanation to the phenomena, a

Entomological Scheimpflug lidar for estimating unique insect classes in-situ field test from Ivory Coast

Acquisition of entomological data with high-frequency lidar is an emerging research field in rapid development. The technique offers very high numbers of observations per time unit, suitable for statistical models. In this work, we use a near-infrared Scheimpflug lidar with a sampling frequency of 3.5 kHz to assess the activity of free flying organisms. In-situ measurements were done during the ra

Acoustically Excited Channel Walls for Microbiological Applications

This work presents a novel concept for acoustofluidic cell manipulation: thin vibrating channel walls. Improved etch recipes enable us to produce thin walls between two microfluidic channels, fabricated in silicon or via PDMS casting, that can be used for challenging microbiological applications. We demonstrate the medium exchange for bacteria utilised for transformation, single-cell rotation for

Cerebral extracellular calcium activity in severe hypoglycemia : Relation to extracellular potassium and energy state

The changes in extracellular Ca2+ (Cae) and K+ (Ke) activities were studied in the rat brain during insulin-induced hypoglycemia. At about the time of onset of isoelectric EEG in severe insulin-induced hypoglycemia (300-g male Wistar rats under 70% N2O anaesthesia), there was an increase in Ke which, at ∼13 mM, was associated with a fall in Cae. Ke peaked at 48 ± 12 mM, and Cae at 0.18 ± 0.28 mM.

Subjectivism and Relational Good

In this paper, a distinctly subjectivist analysis of the nature of relational goodness or goodness for is proposed. Like the generic subjectivist analysis of value, the proposal is to analyse value in terms of attitudes. Specifically, the proposed analysis of goodness for appeals to a special kind of attitude: namely, so-called for-someone’s-sake attitudes. Unlike other analyses in the literature

Renewable energy, energy security and resilient societies

Abstract in UndeterminedSometimes energy security is used as an advocacy for renewable energy systems. Although renewable energy systems can surely have such characteristics they will not per se solve all types of security problems, and new problems will most certainly arise. In this paper, energy security aspects of renewable energy systems are analysed taking the starting point in a broad typolo

Glances and hands on public art : A kaleidoscopic view of monuments in public space in the Öresund region and beyond

How do we relate to monuments and statues in public spaces? And how do our perceptions shift when history confronts us with new insights?In the wake of the Black Lives Matter movement in 2020, many monuments and statues across the world became the focal point of intense debate. Some were torn down, others covered up, and others were re-examined in light of not only colonial and racial oppression,

Value of Information analysis accounting for data quality

Structural Health Monitoring (SHM) can provide valuable information for maintenance-related activities and post-disaster emergency management. However, as with any technological system, SHM systems can be susceptible to errors due to malfunctioning. Therefore, it is essential to assess the potential for malfunctions and the associated costs of maintenance and repair when evaluating the long-term b

SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION

Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of

From space to specs : Requirements for 4MOST

4MOST,1 the 4m Multi-Object spectrographic Survey Telescope, is an upcoming optical, fiber-fed, MOS facility for the VISTA telescope at ESO's Cero Paranal Observatory (Chile). The preliminary design of 4MOST features 2,400 fibers split into a low-resolution (1,600 fibers, 390-900 nm, R > 5; 000) and a high-resolution channel (800 fibers, three arms, ∼20-25 nm coverage each, R > 18; 000) with an Ec

Sjostrom stefan

Microsoft Word - sjostrom_stefan_thesis.doc Forestry Certification: Why it is not leading to sustainable forest management By Stefan Sjöström November, 2003 A thesis submitted in fulfillment of the requirements of the Degree of Master of Science LUMES, Lund University, Sweden Stefan Sjöström Supervisor: Trollebergsvägen 22A Professor Bengt Nihlgård 222 29 Lund Department of Ecology Sweden Sölvegat

https://www.lumes.lu.se/sites/lumes.lu.se/files/sjostrom_stefan.pdf - 2026-05-05

Verksamhetsuppfoljning 2011 0

SingleRespondentReport_Main Strategic Research Environment questionnaire 2011Vinnova Sida 1 av 31 From our application in 2009 the main priorities set were the following for the strategic research area Epidemiology for Health (EpiHealth): - to develop a structure for EpiHealth linking Lund University (LU) and Uppsala University (UU). - to promote excellence in epidemiological research, on a region

https://www.epihealth.lu.se/sites/epihealth.lu.se/files/verksamhetsuppfoljning_2011_0.pdf - 2026-05-05

Capacity to plan for climate change adaptation in the municipality of Svalöv

Klimatförändringar orsakade av antropogena faktorer har en ökande effekt på både naturliga och humana system. Klimatförändringar bidrar till en förändring av varaktigheten, frekvensen och intensiteten av extrema väderhändelser (som till exempel stormar, översvämningar och värmeböljor). Några exempel på konsekvenser från klimatförändringar på de humana systemen är förlust av liv, förlust av egendomThe changing climate caused by anthropogenic factors has an increasing effect on natural and human systems. There is therefore an increasing need for society to adapt to the effects of climate change (IPCC, 2012; Rescalvo et al., 2013) This is vital since the effects of climate change are already being noticed in many parts of the world (Rescalvo et al., 2013). However, municipalities in Sweden ge

Madison & Vine

Titel: Madison & Vine: Hur uppfattar konsumenter produktplacering i film? Seminariedatum: Januari 15, 2015 Kurs: FEKH29, Examensarbete i marknadsföring, 15 högskolepoäng Författare: Alinne Andersson, Lina Shore, Alexander Wegdell Handledare: Jon Bertilsson Nyckelord: Produktplacering, marknadsföring, varumärke, konsumentpåverkan, smygreklam Syfte: Målet med denna uppsats är att djupare undersö