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Plant-based vaccines: novel and low-cost possible route for mediterranean innovative vaccination strategies.

A plant bioreactor has enormous capability as a system that supports many biological activities, that is, production of plant bodies, virus-like particles (VLPs), and vaccines. Foreign gene expression is an efficient mechanism for getting protein vaccines against different human viral and nonviral diseases. Plants make it easy to deal with safe, inexpensive, and provide trouble-free storage. The b

Determination of specific surface by the BET method

Abstract in French On peut déterminer la surface spécifique par des mesures d’adsorption des gaz ou des vapeurs d’après la théorie de Brunauer-Emmett-Teller, que l’on examine ici, de même que les techniques expérimentales disponibles: méthodes statiques (volumétriques ou gravimétriques) et dynamiques plus ou moins complexes. En s’aidant d’exemples, on discute l’application de la théorie de B.E.T. The theoretical background of the adsorption isotherm equation of Brunauer, Emmett and Teller is reviewed, together with the experimental techniques available for measuring physical adsorption of gases by solids. Examples are given to show how such measurements are used to determine the specific surface of constructional materials.

Effects of polyamine pool manipulation on genes expressed during the cell cycle

Popular Abstract in Swedish En cell förökar sig genom delning. Före delningen måste den växa och fördubbla cellinnehållet för att det ska finnas material till två nya celler. En cellcykel är de händelser som sker från det en cell delat sig tills den delar sig igen [Fig 1]. En cellcykel kan delas in i fyra faser som cellen måste genomgå innan den är mogen att dela sig. Under första fasen, G1, tillvThe polyamines - putrescine, spermidine and spermine - are polycations found within cells. They have been shown to be essential for optimal cell proliferation and cell survival. Cells maintain their polyamine levels in a well-regulated system involving biosynthesis, catabolism and uptake/excretion. The biosynthetic enzymes - ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (Ado

Bidragsbedrägeri

This thesis treats allowance fraud. Very probably, allowance fraud is frequent and the authorities have difficulty in stopping it. For that reason, it is important to study the area of allowance fraud. The administrative system of sanctions is compared with the penal system of sanctions against different kinds of allowance fraud relating to social welfare, sickness benefit insurance, and housing a

Ordbetoning och ordbetydelse i rumänska.

Popular Abstract in Swedish Denna avhandling är en dokumentation av mina undersökningar av det rumänska ordet. Jag har som allmänlingvist undersökt några aspekter av det rumänska ordet fonologiskt, fonetiskt och semantiskt. Det finns också mycket skrivet om morfologin i rumänska. Man är väl medveten om att en del suffix tar betoning och andra inte. Men ingen har utnyttjat denna kunskap för att sysThe book is divided into three parts. All three parts dealwith the Romanian word. Part A: Word stress has not been defined in one rule before. I show how one rule can be applied to nouns, verbs and countnouns of Romanian. The principal method is taken from lexical phonology. Part B: Part A gave me the idea to investigate phonological length in the forms of infinitive, 3sg imperfect and perfect. An

Analysis of Response and Development of Permanent Deformation of Unbound Granular Materials Using a Testing Box Technique

Popular Abstract in Swedish Växande krav på transporters kvalitet och kvantitet ger höga förväntningar på vägarnas kvalitet. Därför har omfattande resurser ägnats åt vägbyggnad och särskilt förbättring av befintliga vägar för att bemöta krav på säkerhet, komfort och bärighet. Ett välfungerande vägnät är en viktig komponent i den ekonomi- och samhällsutvecklingsprocess som förbrukar en stor del av The main objective of this thesis is to study the response and development of permanent deformation in unbound granular materials, theoretically and experimentally. A testing box technique is used here to more closely simulate the behaviour of unbound aggregates subjected to external vertical loading. A small box device (800×800×800 mm) is employed to test both the response and permanent deforma

Effects of common polymorphisms in the alpha(1A)-, alpha(2B)-, beta(1)- and beta(2)-adrenoreceptors on haemodynamic responses to adrenaline

Common naturally occurring polymorphisms have been identified in the coding regions of the alpha(1A)-, alpha(2B)-, beta(1)- and beta(2)-adrenoceptor (AR) genes [alpha(1A)-AR R492C, alpha(2B)-AR insertion/deletion (I/D), beta(1)-AR R389G, beta(2)-AR G16R and beta(2)-AR Q27E] and are associated with modified in vivo and in vitro functionality. We tested their possible effects on the haemodynamic res

Evolution of human immunodeficiency virus type 2 coreceptor usage, autologous neutralization, envelope sequence and glycosylation

To investigate why human immunodeficiency virus type 2 (HIV-2) is less virulent than HIV-1, the evolution of coreceptor usage, autologous neutralization, envelope sequence and glycosylation was studied in sequentially obtained virus isolates and sera from four HIV-2-infected individuals. Neutralization of primary HIV-2 isolates was tested by a cell line-based assay and IgG purified from patients'

Little difference between minimum inhibitory concentrations of Mycobacterium tuberculosis wild-type organisms determined with BACTEC MGIT 960 and Middlebrook 7H10.

The MIC wild-type (WT) distribution for Mycobacterium tuberculosis in BACTEC 960 MGIT is not defined, which may result in poor reproducibility for drug susceptibility testing (DST), as several DST methods with different breakpoints are in use. In a comparison between MGIT and Middlebrook 7H10 medium of seven first- and second-line drugs, including 133 MIC determinations of 15 WT isolates, we found

The Process of Verification and Validation of Building Fire Evacuation Models

To date, there is no International standard on procedures and tests to assess the verification and validation (V&V) of building fire evacuation models. Often it is the case that model testers adopt inconsistent procedures, or tests designed for other model uses or they do not test them for all features embedded in their model. For instance, the tests presented within the MSC/Circ.1238 (Guideli

Crystallographic studies on porphyrin metallation by ferrochelatase.

Popular Abstract in Swedish Proteiner är livsnödvändiga och finns i alla celler. De flesta proteinerna katalysear kemiska reaktioner och kallas då enzymer. Enzymer och proteiner (äggviteämnen) är uppbyggda av aminosyror ihopkopplade till kedjor som kan vara olika långa. Det finns 20 stycken olika aminosyror. Man kan göra en liknelse med ett pärlhalsband tillverkat av 20 olika pärlor, varje pärla mFerrochelatase catalyses the terminal step in heme biosynthesis by inserting a ferrous ion into protoporphyrin IX. The reaction mechanism has in this thesis been studied by mainly crystallographic methods. The structure of ferrochelatase from Bacillus subtilis co-crystallized with N-methyl mesoporphyrin allowed for the first time a characterization of the active site of the enzyme. The structure

Breast Cancer Diagnosis Using Extended-Wavelength–Diffuse Reflectance Spectroscopy (EW-DRS) : Proof of Concept in Ex Vivo Breast Specimens Using Machine Learning

This study aims to investigate the feasibility of using diffuse reflectance spectroscopy (DRS) to distinguish malignant breast tissue from adjacent healthy tissue, and to evaluate if an extended-wavelength range (450–1550 nm) has an advantage over the standard wavelength range (450–900 nm). Multivariate statistics and machine learning algorithms, either linear discriminant analysis (LDA) or supporThis study aims to investigate the feasibility of using diffuse reflectance spectroscopy (DRS) to distinguish malignant breast tissue from adjacent healthy tissue, and to evaluate if an extended-wavelength range (450–1550 nm) has an advantage over the standard wavelength range (450–900 nm). Multivariate statistics and machine learning algorithms, either linear discriminant analysis (LDA) or suppor

Quantification Of Glycosaminoglycans In Knee Synovial Fluid From Different Patient Groups And Knee-Healthy Subjects Using High Performance Liquid Chromatography

Purpose: The glycosaminoglycans chondroitin sulfate (CS) and hyaluronic acid (HA) are important for the normal function of articular cartilage, and changes of their sulfation and concentration may play a role in the pathogenesis of osteoarthritis (OA). Therefore, these glycans may be clinically useful as biomarkers in OA management. The purpose of this study was to analyze the CS and HA pattern in

Mimicking Photosystem II with Manganese Model Complexes to Approach Artificial Photosynthesis

Popular Abstract in Swedish Vi går mot ett mer och mer energikrävande samhälle. För att klara ett ökande behov av energi med de begränsande naturresurser som finns på jorden, har ett forskningsprojekt, som denna avhandling är en del av, som mål att utveckla artificiell fotosyntes. Den naturliga fotosyntesen använder sig av solljus för att omvandla koldioxid och vatten till energirika produkter såsThe work in thesis aims for artificial photosyntesis, which could and will mimic natural photosynthesis, the process that uses light to create energy rich compounds from H2O and CO2. Water is oxidized in Photosystem II (PSII) by the Water Oxidizing Complex (WOC), which is a catalytic site on the lumenal side of the thylakoid membrane. The chlorophyll complex P680 absorbs light and electrons are tr

U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner.

B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high